Question
Using a 20 cm long C18 bonded HPLC column filled with 5m size particles and a mobile phase consisting of 20% methanol in water, two
Using a 20 cm long C18 bonded HPLC column filled with 5m size particles and a mobile phase consisting of 20% methanol in water, two pesticides P1 and P2 elute at 9.0 and 9.2 minutes respectively with corresponding base peak widths of 30 and 30.7 seconds respectively. The mobile phase solvent peak elutes at 2 minutes. Calculate: the retention factors (k') for both pesticides the selectivity () the number of theoretical plates (N) and the Height Equivalent to a Theoretical Plate (H) the resolution Rs Identify the parameter (N, k', ) that is responsible for this low resolution and the practical step you would take to improve the separation.
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