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Utilice la Ley de Beer-Lambert para calcular el coeficiente de extincin molar de GFP en el tampn Tris a 397 nm (o el pico que
Utilice la Ley de Beer-Lambert para calcular el coeficiente de extincin molar de GFP en el tampn Tris a 397 nm (o el pico que observ ms cercano a 397 nm).
el pico es A=.023 a una longitud de onda de 397.
GFP es una solucin de 1,5 mg/ml, 0,1 ml
El tampn Tris es 10 mM
Agrega GFP en 1,9 ml de 10 mM de tampn tris.
La longitud del camino es de 1 cm.
Cul es el coeficiente molar usando A=ECL?
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