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Viral inactivation is a crucial step in the purification of recombinant proteins produced by mammalian cells. While microwave treatment can inactivate live viruses it may
Viral inactivation is a crucial step in the purification of recombinant proteins produced by mammalian cells. While microwave treatment can inactivate live viruses it may also damage the protein product. The given table below shoes the first-order inactivation ate constant for virus and protein, as a function of the input level of microwave energy. Mark the level you would use and explain why. At that level how long of an exposure time is needed in order to reproduce the virus content by 10,000 fold? Energy input Kvirus (1/5) Kprotein (1/5) 1 10 100 1x 10-3 5x 10-3 25 x 10-3 125 x 10-3 625 x 10-3 0.01 x 10-3 0.08 x 10-3 0.62 x 10-3 2.08 x 10-3 1.08 x 10-3 1000 10,000
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