1. What is Recombinant DNA (rDNA) technology, and why is it significant in biotechnology?

2. How is rDNA technology used to create genetically modified organisms (GMOs)?

3. What are plasmids, and how are they used in rDNA technology?

4. Explain the process of gene cloning using rDNA technology.

5. What is a restriction enzyme, and what role does it play in rDNA technology?

6. Describe the steps involved in PCR (Polymerase Chain Reaction) and its importance in rDNA technology.

7. How are recombinant DNA molecules inserted into host organisms, and what are the potential applications of this technique?

8. What safety and ethical considerations are associated with rDNA technology and the creation of GMOs?

9. Can you explain the difference between gene therapy and genetic engineering using rDNA technology?

10. What are the benefits and potential risks of using rDNA technology in agriculture and medicine?