What is the volume of chloroform required to decrease the concentration of iron (III) in water (100mI) as [Fe(CNS)]2 at 20ppm concentration level in the presence of selective quaternary ammonium salt ( RaNCl ) to 0.2ppm assuming the distribution ratio of iron (III) complex between chloroform/ water =100. What the extraction percentage will be if the volume of the organic solvent is doubled. A gaseous sample containing mixture of tour phosphorous pessicides namely malathion, dimethoate, chloropyrifos and summicidin was injected under the optimum instrumental operational parameters into GC. Chromatogram shart revealed four well defined symmetric and narrow peaks at 4.3, 5.3, 7.9 and 10.1 min and the corresponding widths of the base line at the four peaks are 0.3.0.5, 0.5 and 0.9cm, for dimethoate, malathion, chlorpyritos and sumnricidin, respecterely. Calculate the values of N, HETP and Rs (Resolution factor) for the separation step assuming that the length of the column used 60cm. What is the concept of TWO of the followings? i. Distribution ratio in liquid-liquid and solid phase extraction -ii. Capacity in adsorption and ion exchange chromatography iii. Chelating agent in metal separation by solvent extraction iv. Leveling and differentiating solvents in chemical equilibria Answer ONE of the followings: i. Discuss one method for calculating the number (N) and the height equivalent to the theoretical plates (HETP) in liquid-solid chromatography. ii. A 90% of gold (iIi) was extracted from an aqueous solution (100 mL ) containing selective chelating agent adjusted to pH56 into n-hexane (5mL) at room temperature after 2min shaking time. Calculate the distribution ratio, D, and what will be the extraction percentage if the volume of n-hexane is doubled. Comment in your data