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When measuring Dissolved Oxygen in seawater for Standardization of the sodium thiosulfate solution, why is it important to to add 50% (v/v) H2SO4, 4 M

When measuring Dissolved Oxygen in seawater for Standardization of the sodium thiosulfate solution, why is it important to to add 50% (v/v) H2SO4, 4 M NaI/8 M NaOH solution, and 3 M MnCl2 in that specific order? What does the yellow color of the solution indicate?

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