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Which of the following statements is FALSE regarding setting up and running a column in column chromatography? o If too much solvent or too polar
Which of the following statements is FALSE regarding setting up and running a column in column chromatography? o If too much solvent or too polar of a solvent is used for wet loading, premature elution of all compounds can occur, resulting in mixed fractions. The column should be as upright as possible to ensure that the bands are as narrow as possible along the length of the column. Failure to pack the stationary phase compactly typically results in air bubbles which then causes channeling and poor separation. All of these statements are true. Failure to add a layer of sand on top of the stationary phase can result in subsequent pitting of the stationary phase surface when solvent/compound is added, resulting in possible band broadening
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