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You are a biochemist working to understand the mechanism of phosphoglycerate mutase (PGM). You are studying the reaction in vitro so that you can follow
You are a biochemist working to understand the mechanism of phosphoglycerate mutase (PGM). You are studying the reaction in vitro so that you can follow the reaction more closely using radioactive phosphorus (32P). Specifically the mutated enzyme you are working with contains a 32P-labeled phosphohistidine in the active site. The outer lying histidine is the location of the mutation (the 2ndHis in the reaction). This second histidine has been converted to an alanine. If you started the reaction to study PGM combining 3-phosphoglycerate (3PG) with 32P labeled enzyme and after the reaction takes place you isolate all of the components. Where would you find the 32P ? Substrate, intermediate, product, enzyme, buffer? Briefly explain why you chose the answer you chose. PGM
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