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You are asked to make 1.0L of a Tricine buffer of pH8.7. The pKa of Tricine is 8.0. The final total concentration of the buffer

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You are asked to make 1.0L of a Tricine buffer of pH8.7. The pKa of Tricine is 8.0. The final total concentration of the buffer is 0.65mM. Recall that the total concentration of a buffer refers to the sum of the concentration of the weak acid and conjugate base forms. (i) First calculate the ratio of conjugate base: weak acid (give your answer to one decimal place). ( 2 points) (ii) Now calculate the concentration of the weak acid form (give your answer to two decimal places, in mM). (I point) (iii) To the above buffer solution, you add 0.1mM of NaOH (strong base, negligible volume). What is the pH of the resulting solution (round off to whole number, no units)? (2 points) (iii) Is the solution in (ii) above still within the buffering range of Bicine? Briefly explain your answer. ( 2 points)

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