You are running a 500L Protein A purification column to purify a 150,000Da monoclonal antibody. Based on data from previous experiments, you know that the association constant between Protein A and this monoclonal antibody is 64M1 and that the dynamic binding capacity of the column is approximately 0.33mM. Historical experience with the column indicates that the elution step is only 85% efficient. If, after loading the column, you measure a concentration of monoclonal antibody in the mobile phase to be 0.02Lg, how much monoclonal antibody (in kg ) should you expect to elute from the column