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You are using culture flasks that have an area of 25 cm2, and have calculated that you have 1.5 million cells in 10 ml of

You are using culture flasks that have an area of 25 cm2, and have calculated that you have 1.5 million cells in 10 ml of DMEM/10% CS, the recommended volume for a 25 cm2 culture flask.

Use that information to calculate the volume needed of your original culture to subculture your cells at a cell density of 10,000 cells/m

Volume of original culture? Volume of medium

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