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You have been given the task to carry out a large - scale batch fermentation process at 1 2 0 0 L bioreactor using a
You have been given the task to carry out a largescale batch fermentation process at
bioreactor using a Saccharomyces cerevisiae strain. It was found from the literature that the
optimal operating conditions to reach steadystate after hours of operation were of
glucose, dissolved oxygen level agitation rate at and aeration rate
of Working volume should be about of the total volume where else ovemight inoculum
size is hours of cultivation time needed to prepare inoculum and seed fermenter.
a Design a stepbystep process scaleup in form of diagram from shake flask up to
production reactor.
b Complete the following table which describes the operating details of your fermentation
schedule.
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