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You have been given the task to carry out a large - scale batch fermentation process at 1 2 0 0 L bioreactor using a

You have been given the task to carry out a large-scale batch fermentation process at 1200L
bioreactor using a Saccharomyces cerevisiae strain. It was found from the literature that the
optimal operating conditions to reach steady-state after 32 hours of operation were 5gL of
glucose, 30C,pH6.5, dissolved oxygen level >10%, agitation rate at 200rpm and aeration rate
of 5Lhr. Working volume should be about 80% of the total volume where else ovemight inoculum
size is 5%(vv).14 hours of cultivation time needed to prepare inoculum and seed fermenter.
a. Design a step-by-step process scale-up in form of diagram from shake flask up to 1200L
production reactor.
b. Complete the following table which describes the operating details of your fermentation
schedule.
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