You have cloned a protein with a C-terminal His6 tag. With the aid of a simple schematic, outline a series of discrete steps that you would use to purify the protein using Ni²⁺ beads. Note that your final protein product MUST be free of the Ni²⁺ beads. On the following pages I have attached a PDF from SepMag that overviews the basics of recombinant protein production and purification. Please review this and make sure that you have an overall understanding of the process.