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You have just gotten back the results from an RNA-seq analysis of mRNAs from liver. You had anticipated counting the number of reads of each
- You have just gotten back the results from an RNA-seq analysis of mRNAs from liver. You had anticipated counting the number of reads of each mRNA to determine the relative abundance of different mRNAs. But you are puzzled because many of the mRNAs have given you results like those shown in figure below. How is it that different parts of an mRNA can be represented at different levels?
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