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You have purchased an antibody against a cell-surface protein and perform a cell-surface titration to estimate that Kd = 3 nM. After labeling your cells
You have purchased an antibody against a cell-surface protein and perform a cell-surface titration to estimate that Kd = 3 nM. After labeling your cells at 500 nM antibody, you wash and resuspend them in antibody-free buffer at room temperature and then leave for lunch. Estimate how many minutes will pass before half of the bound antibody has dissociated from the cells.
Answer is 39 mins
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