You work in a cannabis company and are responsible for analyzing material before it moves into processing for

terpenes and cannabinoids. Some of these components may be present at low concentrations. You are using a non$-$

polar capillary column on a GC$-$FID. Your samples have boiling points ranging from $90$ to $165C.$ Your oven temperature

is set to $195$C$.$ You have an autosampler. Your instrument has an injector range of $50-250$C and the column can go up

to $400C$

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Your work flow is as follows:

Inject a standard containing three of your compounds of interest five times and determine the $\%$RSDs for the

peak areas and retention times found in the chromatogram. The criteria is NLT $5\mathrm{.}0\%$ for each $\%$RSD

calculated.

Inject individual standards

Inject a standard containing all of the analytes

Inject a sample

Perform direct calculations

If you were in method development, what temperature would you set the injector to be$,$ why?

One day you run your replicates and find that the $\%$RSDs are over $7\%,$ based on what we discussed in class,

what do you think might be the problem, why?

Why is it important that we confirm the $\%$RSD for both the peak areas and retention time?

Compound $4$ and Compound $9$ have the same boiling point, explain their retention time differences.

Compound $5$ and Compound $8$ have the same polarity, explain their retention time differences