Your are consulting a start$-$up company and help them assess a chromatographic purification step with a binary protein mixture as feed. Which two parameters would you typically use to quantify how well the two proteins are separated under different conditions? What are these values assuming that the hold$-$up volume is $10$ mL$,$ and the retention times are $3\mathrm{.}44$ min and $3\mathrm{.}98$ min for protein A and B respectively at a linear flow rate of $1\mathrm{.}5$ m h$-1$ and inner column diameter of $0\mathrm{.}016$ m as well as an equal peak width of $1$ mL at the baseline? What is the resolution if the first peak has a baseline width of $2$ mL$?$ What if it was the second peak that had this width?