Your superdrug is showing promise! Your pharmacokinetics results were so inspiring that the company has asked you to look into the hydrolyzation of the drug. Chase-i-cillin is hydrolyzed (and therefore rendered inactive) by the protein chaseicillinase, an enzyme present in some resistant bacteria. The mass of chaseicillinase is 30kd (i.e., the molecular weight is 30 kg/mol). The amount of Chase-i-cillin hydrolyzed in 1 minute in a 10mL solution containing 10-9 g of purified chaseicillinase was measured as a function of the concentration of Chase-i-cillin. Assume that the concentration of Chase-i-cillin does not change appreciably during the assay. Chase-i-cillin (micromolar) Amount hydrolyzed (nanomoles) 1 0.054 3 0.14 5 0.19 10 0.28 30 0.42 50 0.48 a. Plot d[P]/dt versus [S] for these data. Does chase-i-cillin-ase appear to obey Michaelis-Menton kinetics? If so, what is the value of KM?

b. What is the value of vmax? Indicate on the plot how you determined KM and vmax. c. Construct the Lineweaver-Burke plot for this reaction. Be sure to label axes and include units! d. Determine KM and vmax using the Lineweaver-Burke plot. Indicate on the plot how you determined KM and vmax. e. Assume there is one active site per enzyme molecule. What is the turnover number of chaseicillinase under these experimental conditions? f. How much time does it take for the chaseicillinase to hydrolyze one Chase-i-cillin molecule?