Which is the best explanation for the differing effects on reaction rate caused by ascorbic acid at

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Which is the best explanation for the differing effects on reaction rate caused by ascorbic acid at low and high concentrations of JB-76?
(A) At high concentrations of JB-76, there are fewer free active sites for the ascorbic acid to bind to.
(B) At low concentrations of JB-76, there are fewer free allosteric sites for the RZN45 to bind to.
(C) At high concentrations of JB-76, there are more free allosteric sites for the ascorbic acid to bind to.
(D) At low concentrations of JB-76, there are more free active sites for the RZN45 to bind to.


The active site of the enzyme Ritzolinine (RZN45) contains three positively charged lysine residues. When ascorbic acid is present, binding of JB-76, the substrate of RZN45, decreases. The reaction rate is affected by the presence of ascorbic acid as shown in the figure below. It is thought that a daily supplement of Vitamin C might aid those suffering from Ritzolierre's Disease, which is caused by elevated levels of RZN45.Reaction Rate X Ascorbic Acid Present ---- Normal Concentration of JB-76

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