The first reaction in glycolysis is the phosphorylation of glucose:

This is a thermodynamically unfavorable process, with ˆ†Go€² = + 13.8 kJ/mol.
a. In a liver cell at 37°C the concentrations of both phosphate and glucose are normally maintained at about 5 mm each. What would be the equilibrium concentration of glucose-6-phosphate, according to the above data?
b. This very low concentration of the desired product would be unfavorable for glycolysis. In fact, the reaction is coupled to ATP hydrolysis to give the overall reaction

c. What is ˆ†Go' for the coupled reaction?

Transcribed Image Text:

P, glucose glucose-6-phosphate H2O ATP + glucose . → glucose-6-phosphate + ADP H