The half-reactions involved in the lactate dehydrogenase (LDH) reaction and their standard reduction potentials are

Calculate (G at pH 7.0 for the LDH-catalyzed reduction of pyruvate under the following conditions:
(a) [lactate]/[pyruvate] = 1 and [NAD+]/[NADH] = 1
(b) [lactate]/[pyruvate] = 160 and [NAD+]/[NADH] = 160
(c) [lactate]/[pyruvate] = 1000 and [NAD+]/[NADH] = 1000
(d) Discuss the effect of the concentration ratios in parts a-c on the direction of the reaction.

Transcribed Image Text:

Pyruvate + 2H+ + 2 e-→ lactate 。,--0.185 V +H-0.315 V Eor