The hydrolysis of a phenylalanine-containing peptide is catalyzed by a-chymotrypsin with the following results. Calculate KM and

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The hydrolysis of a phenylalanine-containing peptide is catalyzed by a-chymotrypsin with the following results. Calculate KM and Vmax for the reaction.

Peptide Concentration (M)Velocity (M min-1)

2.5 × 10-4.............2.2 × 10-6

5.0 × 10-4.............5.8 × 10-6

10.0 × 10-4.............5.9 × 10-6

15.0 × 10-4.............7.1 × 10-6

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Biochemistry

ISBN: 978-1285429106

8th edition

Authors: Mary K. Campbell, Shawn O. Farrell

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