Question: You are given two preparations of purified pyruvate dehydrogenase complex enzymes with all the required cofactors. You add pyruvate to each preparation and then measure

You are given two preparations of purified pyruvate dehydrogenase complex enzymes with all the required cofactors. You add pyruvate to each preparation and then measure the rate of production of acetyl-CoA and acetaldehyde under aerobic conditions.

How might the pyruvate dehydrogenase complex enzymes differ in each preparation?

Acetyl-CoA (molecules/s) 105 10 Free acetaldehyde Preparation A Preparation B (molecules/s) 10-6 103

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