Question: You are purifying a protein for the first time. You have solubilized it with homogenization in a blender followed by differential centrifugation. You wish to

You are purifying a protein for the first time. You have solubilized it with homogenization in a blender followed by differential centrifugation. You wish to try ammonium sulfate pre-cipitation as the next step. Knowing nothing beforehand about the amount of ammonium sulfate to add, design an experiment to find the proper concentration (% saturation) of ammonium sulfate to use.

Step by Step Solution

★★★★★

3.37 Rating (166 Votes )

There are 3 Steps involved in it

1 Expert Approved Answer

Step: 1 Unlock

Tables exist to tell you how many grams of ammonium sulfate ... View full answer

Question Has Been Solved by an Expert!

Get step-by-step solutions from verified subject matter experts

Step: 2 Unlock

Step: 3 Unlock

Document Format (1 attachment)

481-M-S-B-C (121).docx

120 KBs Word File

Students Have Also Explored These Related Biochemistry Questions!

For the first time in two years, Big G (the cereal division of General Mills) raised cereal prices by 2 percent. If, as a result of this price increase, the volume of all cereal sold by Big G dropped...

In 2012, the FOMC stated for the first time that it aims at an inflation rate of 2 percent (based on the price index of personal consumption expenditures). How might this announcement help secure...

You are auditing for the first time the financial statements of Central Savings and Loan Association for the year ending December 31, 20X3. The CPA firm that audited the associations financial...

1 You decide to heterologously express and purify a recombinant protein, KU 1 5 3 , to assess its' therapeutic potential. KU 1 5 3 has a relative molecular weight of 6 4 kDa , a pl of approximately 7...

You need a buffer at pH 7.5 for use in purifying a protein at 4C. You have chosen Tris, pK 8.08, = H = 50 kJ mol-1. You carefully make up 0.01 M Tris buffer, pH 7.5 at 25C, and store it in the cold...

Details of a mixture of three proteins are below. Protein A: 49,198 Da. pI= 4.71 Protein B: 15,273 Da. pI= 10.8 Protein C: 85,019 Da. pI= 7.26 a) At pH=8, which protein will elute first in an anion...

which method would be the least reliable and why? - Dye binding method (bradford method) of protein determination -bicinchoninic acid (bca) method for protein determination - lowry method of protein...

Can someone please help me with questions? It just has a few sub-parts; I already did 1a. Can you help me with 2-4? I have uploaded my data and graph to help. I already did the calculations, just...

(30 pts) You are working on manufacturing a protein important for a therapeutic treatment. In manufacturing the protein, you have a toxic by-product that is created. In the past, removing this toxic...

You are tasked with purifying two integral membrane proteins from HEK293 cells: C4: KDELR1, KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 1(-29 kDa) D11: Corf105,...

Match the column Column I 20% by volume aqueous CH5OH solution (P) by volume, having density of 0.96 g cm- (A) 5V HO solution. 18 M aq HSO4 solution, having density of 1.84 g cm-3 20% aq. HSO4...

Duroc Jersey pigs are typically red, but a sandy variation is also seen. When two different varieties of true-breeding sandy pigs were crossed to each other, they produced F, offspring that were red....

Whats the difference between a website and a web application?

Criticise the following statistical reasoning. The average price of a dwelling is 54 150. The average mortgage advance is 32 760. So purchasers have to find 21 390, that is, about 40% of the purchase...

Of what benefit is it for DNA to have thymine rather than uracil?

Describe the excision repair process in DNA, using the excision of thymine dimers as an example.

How does proofreading take place in the process of DNA replication?

Question 28 According to a study done by a university student, the probability a randomly selected individual will not cover his or her mouth when sneezing is 0.267. Suppose you sit on a bench in a...

Ch 04- Blueprint Problems - Analysis of Financial Statements Net plant and equipment. Total assets Accounts parable Accruals Notes payable Total current liabilities. Long-term debt Common stock...

The accompanying table lists the ages of acting award winners matched by the years in which the awards were won. Construct a scatterplot, find the value of the linear correlation coefficient r, and...