Question: Electrospray ionization mass spectrometry (ESI-MS) of proteins involves creating positively charged ions of the protein and separating them according to their mass-to-charge ratio (mz). (a)

Electrospray ionization mass spectrometry (ESI-MS) of proteins involves creating positively charged ions of the protein and separating them according to their mass-to-charge ratio (mz).
(a) What causes the different positive charges on different particles of the protein?
(b) The amino acid composition (in numbers of residues per chain) of hen egg-white lysozyme (HEWL) is as follows:

What is the maximum positive charge that can be present on a HEWL ion?

12363 HEFVQ 4 18167 2 NLGKT 32166 YMRIW 2 27811 PDCAS

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