Question: You wish to determine the sequence of a polypeptide that has the following amino acid composition. (a) What is the maximum number of peptides you

You wish to determine the sequence of a polypeptide that has the following amino acid composition.

(a) What is the maximum number of peptides you can expect if you cleave the polypeptide with cyanogen bromide?
(b) What is the maximum number of peptides you can expect if you cleave the polypeptide with chymotrypsin?
(c) Analysis of the intact polypeptide reveals that there are no free sulfhydryl groups. How many disulfide bonds are likely to be present?
(d) How many different arrangements of disulfide bonds are possible?

1 Ala 4 Arg 2 Asn 3 Asp 4 Cys 3 Gly Gln 4 Glu 1 His 1 Lys 1 Met 1 Phe 2 Pro 4 Ser 2 Tyr Trp

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