Initial rate data for an enzyme that obeys Michaelis-Menten kinetics are shown in the following table. When the enzyme concentration is 3 nmol ml-1, a Line weaver-Burk plot of this data gives a line with a y-intercept of 0.00426 (μmol-1ml s).

a. Calculate kcat for the reaction.
b. Calculate KM for the enzyme.
c. When the reactions in part (b) are repeated in the presence of 12 μM of an uncompetitive inhibitor, the y -intercept of the Line weaver-Burk plot is 0.352 (μmol-1 ml s). Calculate K'I for this inhibitor.

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[S] μΜ 320 160 80.0 40.0 20.0 10.0 169 132 92.0 57.2 32.6 17.5