Suppose that you centrifuged a transfer RNA molecule to equilibrium in an alkaline CsCl gradient, as described
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Suppose that you centrifuged a transfer RNA molecule to equilibrium in an alkaline CsCl gradient, as described in Problem 7. What result would you expect?
Problem 7.
For some DNAs, it is possible to separate the two strands, after denaturation, in a CsCl gradient.
(a) What property of any DNA determines where it will band in a CsCl gradient?
(b) What kind of DNA might have two strands that differ sufficiently in this property that they could be separated after denaturation?
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Related Book For
Biochemistry Concepts And Connections
ISBN: 9780134641621
2nd Edition
Authors: Dean Appling, Spencer Anthony-Cahill, Christopher Mathews
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