Effects of temperature on ethanol production. A lowcost and highly productive bio-fuel production method is high-temperature fermentation. However, heat stress can inhibit the amount of ethanol produced during the process.

In Engineering Life Sciences (Mar. 2013), biochemical engineers carried out a series of experiments to assess the effect LO4 of temperature on ethanol production during fermentation.

The maximum inhibitory concentration of ethanol (grams per liter) was measured at four different temperatures (30°, 35°, 40°, and 45° Celsius). The experiment was replicated 3 times, with the data shown in the table. (Note: The data are simulated based on information provided in the journal article.) Do the data indicate that high temperatures inhibit mean concentration of ethanol? Test using a = .10.

Temperature 30° 35° 40° 45°

103.3 101.7 97.2 55.0 103.4 102.0 96.9 56.4 101.0 101.1 96.2 54.9