Iron in blood serum can be measured by reducing it to Fe 2+ and reacting it with
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Iron in blood serum can be measured by reducing it to Fe2+ and reacting it with ferrozine to form [Fe(ferrozine)3]4–, a purple complex that has a maximum absorbance at 562 nm.
The molar absorption coefficient, ε, for the complex at 562 nm is 2.79 * 104 L · mol–1 · cm–1. A solution of the complex ion had an absorbance at 562 nm of 0.703 in a cuvette with path length 2.00 cm. Determine the molar concentration of [Fe(ferrozine)3]4– in the solution.
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Chemical Principles The Quest For Insight
ISBN: 9781464183959
7th Edition
Authors: Peter Atkins, Loretta Jones, Leroy Laverman
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