At room temperature sucrose is hydrolyzed by the catalytic action of the enzyme sucrase as follows:

Starting with a sucrose concentration C_A0 = 1.0 millimol/liter and an enzyme concentration CEO = 0.01 millimol/liter, the following kinetic data are obtained in a batch reactor (concentrations calculated from optical rotation measurements):

Determine whether these data can be reasonably fitted by a kinetic equation of the Michaelis-Menten type, or

If the fit is reasonable, evaluate the constants k₃ and C_M. Solve by the integral method.

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sucrose sucrase products