One method for quantitative determination of the concentration of constituents in a sample analyzed by gas chromatography
Question:
One method for quantitative determination of the concentration of constituents in a sample analyzed by gas chromatography is the area normalization method. In this method, complete elution of all the sample constituents is necessary. The area of each peak is then measured and corrected for differences in detector response to the different eluates. This correction is accomplished by dividing the area by an empirically determined correction factor. The concentration of the analyte is found from the ratio of its corrected area to the total corrected area of all peaks. For a chromatogram containing three peaks, the relative areas were found to be 16.4, 45.2, and 30.2 in the order of increasing retention time. Calculate the percentage of each compound if the relative detector responses were 0.60, 0.78, and 0.88, respectively.
Step by Step Answer:
Fundamentals Of Analytical Chemistry
ISBN: 9780357450390
10th Edition
Authors: Douglas A. Skoog, Donald M. West, F. James Holler, Stanley R. Crouch