Consult Table 5-1 to complete the following:

(a) On a plot of absorbance at 280 nm versus elution volume, sketch the results of gel fi ltration of a mixture containing human cytochrome c and bacteriophage T7 RNA polymerase and identify each peak.

(b) Sketch the results of SDS-PAGE of the same protein mixture showing the direction of migration and identifying each band.

Table 5.1

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Protein Proteinase inhibitor III (bitter gourd) Cytochrome c (human) Myoglobin (horse) Interferon-y (rabbit) Chorismate mutase (Bacillus subtilis) Triose phosphate isomerase (E. coli) Hemoglobin (human) RNA polymerase (bacteriophage T7) Nucleoside diphosphate kinase (Dictyostelium discoideum) Pyruvate decarboxylase (yeast) Glutamine synthetase (E. coli) Titin (mouse) Amino Acid Residues 30 104 153 288 381 510 574 883 930 2,252 5,616 35,213 Subunits 1 1 1 2 3 2 4 1 6 4 12 1 Protein Molecular Mass (D) 3,427 11,617 16,951 33,842 43,551 53,944 61,986 98,885 100,764 245,456 621,264 3,906,488