For the experimental system described in Problem 17, and assuming all the substrates for oxidative phosphorylation are present in excess, does ATP synthesis increase or decrease with an increase in the pH of the fluid in which the submitochondrial particles are suspended?

Problem 17

Bombarding a suspension of mitochondria with high-frequency sound waves (sonication) produces submitochondrial particles derived from the inner mitochondrial membrane. These membranous vesicles seal inside out, so that the intermembrane space of the mitochondrion becomes the lumen of the submitochondrial particle. Diagram the process of electron transfer and oxidative phosphorylation in these particles.