An experiment was conducted in which a substrate concentration S (in M) was varied and a reaction
Question:
An experiment was conducted in which a substrate concentration S (in M) was varied and a reaction rate V (in M/s) was measured for each value of S. The following (pretend) data were obtained.
The data are expected to follow the equation V(S) = Vm S 3 K3 + S 3 .
Using the experimental data, follow the steps below to determine the values of K and Vm.
a. Linearise the equation by writing 1 V as a function of 1 S 3 .
b. Compare the linearised equation to the equation of a line y = mx +
b, and express m and b in terms of K and Vm.
c. Express K and Vm in terms of the slope and y intercept.
d. Construct a table of values for x and y by appropriately modifying the experimental data.
e. Plot the modified experimental data and construct a line through these two points. Calculate the values of m and b for this line.
f. Calculate K and Vm using the slope and y intercept of the line calculated above.
Step by Step Answer:
Mathematics And Statistics For Science
ISBN: 9783031053177
1st Edition
Authors: James Sneyd, Rachel M. Fewster, Duncan McGillivray