Escherichia coli cells are grown for many generations in a medium in which the only available nitrogen is the heavy isotope 15 N They are then transferred to a medium containing 15 N as the only source of nitrogen (a) What distribution of 15 N and 14 N would be expected in the DNA molecules of cell...

a i Onehalf of the DNA molecules with 15 N in both strands a ...

Escherichia coli cells are grown for many generations in a medium in which the only available nitrogen

Question:

Escherichia coli cells are grown for many generations in a medium in which the only available nitrogen is the heavy isotope ¹⁵N. They are then transferred to a medium containing ¹⁵N as the only source of nitrogen.
(a) What distribution of ¹⁵N and ¹⁴N would be expected in the DNA molecules of cells that had grown for one generation in the ¹⁴N-containing medium assuming that DNA replication was

(i) conservative,

(ii) semiconservative,

(iii) dispersive?

(b) What distribution would be expected after two generations of growth in the 14N-containing medium assuming

(i) conservative,

(ii) semiconservative, or

(iii) dispersive replication?

Distribution
The word "distribution" has several meanings in the financial world, most of them pertaining to the payment of assets from a fund, account, or individual security to an investor or beneficiary. Retirement account distributions are among the most...

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