(a) Why are HPLC particles porous? (b) Why are particles with 60- to 120- pores used for...

Question:

(a) Why are HPLC particles porous?
(b) Why are particles with 60- to 120-Å pores used for small molecules but wide-pore 300-Å stationary phases used to separate polypeptides and proteins?

Fantastic news! We've Found the answer you've been seeking!

Step by Step Answer:

Related Book For book-img-for-question

Quantitative Chemical Analysis

ISBN: 9781464135385

9th Edition

Authors: Daniel C. Harris

See More Books

Question Posted: Jun 24, 2021 08:05 AM

See More Questions

Why are silica stationary phases generally limited to operating in the pH range 2-8? Why does the silica in Figure 24-8 have improved stability at low pH?
Why are gas chromatographic stationary phases often bonded and cross-linked? What do these terms mean?
Porous canvas bags filled with water are used by travelers in hot weather. When the bags are slung on the outside of a fast-moving car, the water inside is cooled considerably. Explain.
Derive the velocity potential for a doublet; i.e., derive Eq. (3.88).
What are some of the predictable consequences of a trust gap?
Knowing that the tension is 850 N in cable AB and 1020 N in cable AC, determine the magnitude and direction of the resultant of the forces exerted at A by the two cables. G00 ann
A random sample of 150 observations was classified into the categories shown in the table below. LM8-5l.DAT...
In the Chapter 3 Data Analytics Case, you applied Tableau to examine a data set and create calculations to compare two companies profitability. For the case in this chapter, you continue in your role...
Required a. For each of the seven distinct parts of the standard unqualified report prepared in accordance with generally accepted auditing standards in the United States, describe whether key...
Suppose that the market for black sweaters is a competitive market. The following graph shows the daily cost curves of a firm operating in this market. For each price in the following table,...
(a) Why is high pressure needed in HPLC? (b) For a given column length, why do smaller particles give a higher plate number? (c) What is a bonded phase in liquid chromatography?
How do additives such as triethylamine reduce tailing of certain solutes?
If the point (1 - a, b + 1) lies on the graph of an even function f, then what does f(a - 1) equal?
From the following electrochemical cells write: (a) the electrode half reactions for oxidation and reduction (b) the overall cell reaction (c) the NERNST equation for the emf for each of the cells...
Use the range rule of thumb to identify the values that are significantly low, the values that are signficantly high, and the values that are neither significantly low nor significantly high. A test...
On January 1, Year 1, Handy Company (Handy) purchased 70% of the outstanding common shares of Dandy Limited (Dandy) for $11,900. On that date, Dandys shareholders equity consisted of common shares of...
CarFind Inc. showed the following equity information at December 31, 2019. Common shares, unlimited shares authorized; 4,900,000 shares issued and outstanding Retained earnings $1,045,000 1,385,000...
Cell Phone Radiation Listed below are the measured radiation rates (in W/kg) corresponding to these cell phones: iPhone X, iPhone 7, Google Pixel, Samsung Galaxy 58, OnePlus 5, Motorola VE465, LG G3,...
This problem continues our accounting for Fitness Equipment Doctor, Inc., from Chapter 9. Fitness Equipment Doctor, Inc., has been authorized to sell 100,000 shares of $5 par value common stock and...
Find an equation of the given line. Slope is -2; x-intercept is -2
What is the density of 53.4 wt% aqueous NaOH (FM 40.00) if 16.7 mL of the solution diluted to 2.00 L gives 0.169 M NaOH?
How many milliliters of 3.00 M H2SO4 are required to react with 4.35 g of solid containing 23.2 wt% Ba(NO3)2 if the reaction is Ba2++ SO24 BaSO4(s)?
How many grams of 0.491 wt% aqueous HF are required to provide a 50% excess to react with 25.0 mL of 0.0236 M Th4+ by the reaction Th4+ + 4F- n ThF4(s)?
Which of the following accounts will not be closed during the closing process? a. Accounts Recelvable b. Wages Expense c. Fees Earned d. Rent Expense
Clarkson Lumber Company After a rapid growth in its business during recent years, the Clarkson Lumber Company, in the spring of 1996, anticipated a further substantial increase in sales. Despite good...
How do external factors such as changing consumer preferences affect the retail industry?"

(a) Why are HPLC particles porous? (b) Why are particles with 60- to 120- pores used for...

Question:

Step by Step Answer:

AThe particles used for the HPLC separation of molecule should have pores that are sufficiently larg...View the full answer

Quantitative Chemical Analysis

Students also viewed these Sciences questions