Human serum albumin (HSA) is an important protein ingredient in cryopreservation media used in procedures such as in vitro fertilization. Search the literature for a highperformance liquid chromatography method for the determination of human serum albumin and the stabilizer N-acetyltryptophan in medical devices.

(a) Give the citation (authors, title, journal name, year, volume, pages) for the research paper that fi ts the criteria of this analysis.

(b) What alternative methods could be used for analysis of human serum albumin?

(c) What type of analytical column is used for the separation?

(d) How long was the gradient? How long were the additional wash and equilibration steps within the gradient method?

(e) What parameters were assessed in the method validation?

(f) Why were particles with 300 Å pores used?