Compound P, which absorbs light at 305 nm, was titrated with X, which does not absorb at
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Compound P, which absorbs light at 305 nm, was titrated with X, which does not absorb at this wavelength. The product, PX, also absorbs at 305 nm. Absorbance was measured in a 1.000-cm cell, and the concentration of free X was determined independently, with results in the table. Prepare a Scatchard plot and find the equilibrium constant for the reaction X + P ⇌ PX.
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Po Xo (М) [X] (M) Experiment (М) A 0.010 0 0.213 4.42 x 10 9.10 X 10 1.60 X 10 2.47 X 10 3.57 X 10 1 0.010 0 0.001 00 0.303 2 0.010 0 0.002 00 0.394 -5 3 0.010 0 0.003 00 0.484 -5 4 0.010 0 0.004 00 0.575 0.010 0 0.005 00 0.663 0.010 0 0.006 00 0.752 5.52 X 10 -5 7 0.010 0 0.007 00 0.840 8.20 X 10 -4 1.42 X 10 2.69 X 10 5.87 X 10 9.66 X 10 8 0.010 0 0.008 00 0.926 -4 9. 0.010 0 0.009 00 1.006 -4 10 0.010 0 0.010 00 1.066 -3 11 0.010 0 0.020 00 1.117
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