Lee Hartwell isolated a mutant allele of CDC28 that resulted in the inability of haploid yeast cells, which have 16 chromosome and 12 X10^6 base pairs, tom arrest prior to entry into S-phase of the cell cycle. Using FACS analysis, what would you expect the fluorescent profile to look like on the isolated mutant cells?

A) Even peaks of 2C and 4C DNA content

B) One peak of 2C DNA content

C) A broad peak from 2C to 4C content

D) A single peak at 1C DNA content

E) No fluorescent signal because the cells could not replicate their DNA