1. a) A volume of 10L of a crude enzyme extract from a liver tissue was found to catalyze a reaction at a rate of 0.10moles/min under optimal assay conditions. Following fractionation of 50mL of the extract by ammonium sulfate precipitation, the resulting precipitate was re-dissolved in 10mL of buffer. Using 10L of the purified extract, the reaction rate was found to be 0.18moles/min. Calculate the percentage recovery of the enzyme. b) In a second purification step, the entire mixture of the purified extract is subjected to chromatography on Q-Sepharose. Elution using 200mMNaCl/ buffer yields a 5mL fraction with a measured reaction rate of 0.30moles/min(10L were used in the assay). Calculate the percentage recovery of the enzyme for the second purification step. c) Based on the answers to a) and b), which purification step(s) could benefit from improvement (and how)