• 1 Frances Redi disproved spontaneous generation by designing a controlled experiment where he placed raw meat in six (6) jars. He covered three jars with a fine net and left three jars open. After a short period, maggots appeared in the open jars while the covered jars had no maggots. In your opinion, what additional controls and measurements could Frances Redi have done to prove that rotting of raw meat is caused by live bacteria?
• 2) Microorganisms were inoculated on two agar plates and incubated for 24 hours. After incubation, no microbial colonies were seen on the spread plate agar, but three microbial colonies were seen in the pour plate agar. What may account for this observation and how can growth of this microorganism be enhanced?                                             

3) Discuss three importance of glycocalyx to microorganisms with specific examples.