Answered step by step
Verified Expert Solution
Question
1 Approved Answer
2. (15) Enzyme kinetics - multiple substrates. Certain enzymes mediate catalysis of multiple substrates. Assume that a single enzyme E catalyzes the conversion of two
2. (15) Enzyme kinetics - multiple substrates. Certain enzymes mediate catalysis of multiple substrates. Assume that a single enzyme E catalyzes the conversion of two different substrates S, and S, to two different products P, and P2, as follows: ki E+S Es, k3 E + P (Eq. 1) K-1 E + S2 ES2 kg E + P2 (Eq. 2) 81 (a) (11) Assume that the initial enzyme concentration [E.] is known and that the quasi-steady state assumption holds true for [ES] and [ES2]. Derive the expression for the product formation rate vi for P, in terms of rate constants, [E.], [Si], and [S2]. You must show the steps of the derivation, not just the final answer. (b) (4) Based on what you found in part (a), decide if the following statements are true or false: a. The maximum production rate of P, will not change by having two substrates. b. By having the second substrate S2, the apparent Michaelis-Menten constant Km.app for v; increases
Step by Step Solution
There are 3 Steps involved in it
Step: 1
Get Instant Access to Expert-Tailored Solutions
See step-by-step solutions with expert insights and AI powered tools for academic success
Step: 2
Step: 3
Ace Your Homework with AI
Get the answers you need in no time with our AI-driven, step-by-step assistance
Get Started