Answered step by step
Verified Expert Solution
Link Copied!

Question

1 Approved Answer

24.7 You are interested in purifying a sample containing alcohol dehydrogenase from horse liver. However, the sample also contains the protein catalase. These two proteins

image text in transcribed
24.7 You are interested in purifying a sample containing alcohol dehydrogenase from horse liver. However, the sample also contains the protein catalase. These two proteins have the following transport properties: S(s) 11.3x10-13 4.88x10-13 D (m-/s) 4.1x10-11 6.5x10-11 V (cm'/g) 0.715 0.751 Catalase Alcohol dehydrogenase Determine the molecular weight of catalase and alcohol dehydrogenase b. You have access to a centrifuge that can provide angular velocities up to 35,000 rpm. For the specieis you expect to travel the greatest distance in the centrifuge tube, determine the time it would take to centrifuge until a 3 cm displacement of the boundary layer occurs relative to the initial 5 cm location of the boundary layer relative to the centrifuge axis. c. To separate the proteins, you need a separation of at least 1.5 cm between the boundary layers associated with each protein. Using your answer in part b, will it be possible to separate the proteins by centrifugation

Step by Step Solution

There are 3 Steps involved in it

Step: 1

blur-text-image

Get Instant Access to Expert-Tailored Solutions

See step-by-step solutions with expert insights and AI powered tools for academic success

Step: 2

blur-text-image

Step: 3

blur-text-image

Ace Your Homework with AI

Get the answers you need in no time with our AI-driven, step-by-step assistance

Get Started

Recommended Textbook for

Transportation A Global Supply Chain Perspective

Authors: John J. Coyle, Robert A. Novak, Brian Gibson, Edward J. Bard

8th edition

9781305445352, 1133592961, 130544535X, 978-1133592969

More Books

Students also viewed these Mathematics questions