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4. Chromatograms of compounds A and B were obtained at the same flow rate with two columns of equal length. The value of tm is
4. Chromatograms of compounds A and B were obtained at the same flow rate with two columns of equal length. The value of tm is 1.3min in both cases. Interpret the chromatogram below. Use the fill-in-the-blanks. ( 21 marks) a) Column 1 (1 or 2 ) has a smaller plate height. This is because the equation (larger/smaller)variance,meansH , where the smaller plate height means (broaderarrower) peaks. b) Column 1 ( 1 or 2 ) has more theoretical plates. This is because c) Column (1 or 2) has lower resolution. This is because d) The relative retention of column 1(>/=/
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