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4. Consider the enzyme-catalyzed conversion of substrate A to product Z. Experiments were performed in a 1 cm, 1 mL cuvette. Extinction coefficient of substrate

4. Consider the enzyme-catalyzed conversion of substrate A to product Z. Experiments were performed in a 1 cm, 1 mL cuvette. Extinction coefficient of substrate A = 4,800 M-1cm-1.

You find that the KM for substrate A = 65 M. What is the VMAX value in M/min if the measured increase in absorbance at 475 nm is 0.15/min when [substrate A] = 1.0 mM (Keep Beers Law in mind for this calculation).

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