4. You are working on a research project and have identified two inhibitors, A and B of an enzyme.You measure the initial rates of the enzyme catalyzed reaction in the absence of inihibitor, in the presence of 8.0 mM A, and 8.0 mM B. The data are tabulated below.

[S]/[M]	No inhibitor v0 /Ms-1	8.0 mM Inhibitor A v0 /Ms-1	8.0 mM Inhibitor B v0 /Ms-1
5 x 10-4	1.25 x 10-6	5.8 x 10-7	3.8 x 10-7
1.0 x 10-3	2.0 x 10-6	1.04 x 10-6	6.3 x 10-7
2.5 x 10-3	3.13 x 10-6	2.00 x 10-6	1.00 x 10-6
5.0 x 10-3	3.85 x 10-6	2.78 x 10-6	1.25 x 10-6
1.0 x 10-2	4.55 x 10-6	3.57 x 10-6	1.43 x 10-6

a) Determine the values of Km and Vmax of the enzyme.

b) Determine the type of inhibition imposed by inhibitors A and B, and calculate KI for each inhibitor.

Show all work including plots and calculations.