Question
4) You have a DNA fragment that has been produced by cutting a DNA molecule with Sau3AI, and the plasmid is digested with BamHI. a)
4) You have a DNA fragment that has been produced by cutting a DNA molecule with Sau3AI, and the plasmid is digested with BamHI.
a) Taking into account the cutting sequences of the aforementioned restriction enzymes, is it possible that they can bind by ligation? Explain your answer.
b) If ligation is possible, can the cloned vector be cut again with BamHI and the original fragment cut at its ends with Sau3AI?
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a Each restriction enzyme recognizes a short specific sequence of nucleotide bases t...
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Elementary Principles of Chemical Processes
Authors: Richard M. Felder, Ronald W. Rousseau
3rd Edition
978-0471687573, 9788126515820, 978-0-471-4152, 0471720631, 047168757X, 8126515821, 978-0471720638
