A small DNA molecule was cleaved with several dierent restriction nucleases, and the size of each fragment
Question:
Enzyme Fragment Size (kb)
Eco RI ................................. 1.3, 1.3
Hpa II ..................................... 2.6
Hind III ................................... 2.6
Eco RI + Hpa II ............... 1.3, 0.8, 0.5
Eco RI + Hind III ............ 0.6, 0.7, 1.3
(a) Is the original molecule linear or circular?
(b) Draw a map of restriction sites (showing distances between sites) that is consistent with the data given.
(c) How many additional maps are compatible with the data?
(d) What would have to be done to locate the cleavage sites unambiguously with respect to each other?
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Related Book For
Biochemistry Concepts and Connections
ISBN: 978-0321839923
1st edition
Authors: Dean R. Appling, Spencer J. Anthony Cahill, Christopher K. Mathews
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