The following diagram shows one-half of a restriction site.
(a) Draw the other half.

(b) Use heavy arrows (†‘†“) to identify type II cleavage sites that would yield blunt-ended duplex DNA products.
(c) Use light arrows (†‘†“) to identify type II cleavage sites yielding staggered cuts that could be converted directly to recombinant DNA molecules by DNA ligase, with no other enzymes involved.
(d) If this were the recognition site for a type I restriction endonuclease, where would the duplex be cut?
(e) If DNA sequences were completely random, how large an interval (in kilobase pairs) would you expect on average between identical copies of this sequence in DNA?

Transcribed Image Text:

G A C G T C